Dual-luciferase reporter assay showed that miR-140-3p significantly decreased the luciferase activity of report genes containing wild-type BRD4 (wt-BRD4), while there was no effect on the mutated 3’-UTR BRD4 (mut-BRD4) in ACC cells (figure 7f). This evidence concerns the gene BRD4 and adrenal cortex carcinoma.