In addition, as MC4RKO mice show enhanced appetites and obesity as well as increased expression of IGF-1 in the liver to decelerate steatohepatitis progression (Nagoya et al., 2020), IGF-1 might contribute to the milder increase in transaminase levels seen in MC4RKO mice than in other mouse models, in the early phase of the disease (Table S4). This evidence concerns the gene IGF1 and obesity due to melanocortin 4 receptor deficiency.