We also examined the contribution of central 5-HT in the brain as it plays a role in appetite control as a neurotransmitter along with its receptor HTR2C via the MC4R signaling pathway (Namkung et al., 2015; Lam et al., 2010), independently of the 5-HT in the SI; however, no study has described the kinetics of central 5-HT secretion and its relationship with peripheral 5-HT in NAFLD. This evidence concerns the gene HTR2C and metabolic dysfunction-associated steatotic liver disease.