Additionally, no significant changes were observed in the expression of genes involved in fatty acid oxidation (Cpt1a), very low-density lipoprotein (VLDL) secretion (Mttp), lipogenic transcription factors (Pparg and Srebf1) (data not shown), inflammation (Adgre1 and Ccl2) or fibrosis (Col1a1, Tgfb1 and Timp1) (Fig. 4), suggesting that suppression of 5-HT and Htr2a expression by visceral nerve blockade inhibits fatty acid synthesis in the liver of diet-induced NAFLD mice. This evidence concerns the gene TGFB1 and metabolic dysfunction-associated steatotic liver disease.