Then, we analyzed the expression of 5-HT, HTRs and lipid metabolism-related genes using diet-induced and central neuron-dependent murine NAFLD models of MC4RKO mice (Nagoya et al., 2020; Itoh et al., 2011) with or without neural signal blockade of the liver-gut autonomic neural axis. The gene discussed is HTR5A; the disease is metabolic dysfunction-associated steatotic liver disease.