To fully address the effects of AEP cleavage of Tmod3 on GBM progression in vivo, nude mice orthotopic glioma model using the constructed U87-MG cells (NC, AEP KD, AEP KD/tTmod3-C res, AEP KD/tTmod3-N res and AEP KD/tTmod3-N res/tTmod3-C res) were established. Here, TMOD3 is linked to central nervous system cancer.