The following RT–qPCR data illustrated that hypoxia treatment led to an increase in DACT3-AS1 expression, while downregulation of HIF-1α hindered this induction, which suggested that the expression of DACT3-AS1 was regulated by HIF-1α in hypoxic HCC cells (Fig. 1c). The gene discussed is HIF1A; the disease is hepatocellular carcinoma.