After testing the overexpression efficiency of pcDNA3.1-FOXA3 (Supplementary Fig. 5b), we further detected the RNA and protein levels of PKM2 in response to FOXA3 overexpression and found that FOXA3 augmentation reduced the expression of PKM2 in HCC cells (Fig. 8b–c). The gene discussed is FOXA3; the disease is hepatocellular carcinoma.