In line with the variations observed in C-ALD and AMN patient’s fibroblasts, Abcd1 KO glial cells exhibited an increase of C24:0 (4.9-fold, P < 0.001, Fig. 3A) and C26:0 (17.5-fold, P < 0.0001, Fig. 3A), and no variation in C22:0 levels, compared with wild type (wt) mouse-derived cells. This evidence concerns the gene ABCD1 and adrenomyeloneuropathy.