We found that a high rate of glutamine uptake at early phases of macrophage infection (up to 8 hpi) (Fig. 4A) coincided with M1-like polarization (refer to Fig. 1) and with increased mRNA molecules of the Gls gene (Fig. 4B), which encodes the mitochondrial glutaminase (GLS), a key enzyme in the glutaminolysis pathway. Here, GLS is linked to infection.