To confirm whether this phenomenon occurs in both hemizygous and heterozygous DMD iPSC-CMs, we analyzed the mRNA levels, and protein expression of the cardiac ion channels NaV1.5 (encoded by SCN5A gene), Kir2.1 (encoded by KCNJ2 gene), and CaV1.2 (encoded by CACNA1C gene). The gene discussed is SCN5A; the disease is Duchenne muscular dystrophy.