MTOR and breast carcinoma: Indeed, in all breast cancer cell lines, both drugs determined the inhibition of phospho-mTOR protein expression, paralleled to a significant induction of autophagy, as shown by western blot analysis and quantification of the LC3-I conversion to the lipidated, autophagosome-associated form, LC3-II (Fig. 2 and Additional file 1: Fig. S1c).