Next, we examined TMPO-AS1 expression in a panel of ESCC cell lines and two normal esophageal epithelial cell lines (Het1A and NE1) and found that the TMPO-AS1 level was significantly higher in the tumor cell lines than in normal cell lines (Supplementary Fig. 1h). The gene discussed is TMPO; the disease is esophageal squamous cell carcinoma.