TMPO and esophageal squamous cell carcinoma: We conducted a series of rescue experiments to investigate whether TMPO-AS1 performs its function in ESCC by regulating TMPO. Consistent with our prediction, MTS and Transwell assays showed that TMPO overexpression in TMPO-AS1-silenced cells decreased the inhibition of cell proliferation, G1/S progression, migration and invasion (Fig. 4f-h, Supplementary Fig. 4h).