Collectively, these results reveal that TMPO-AS1 recruits FUS/p300 to the TMPO promoter and forms biomolecular condensates by direct binding, promoting H3K27ac and facilitating the transcription of TMPO, resulting in subsequent upregulation of CyclinD1 and MTA1, ultimately leading to ESCC tumor development. Here, CCND1 is linked to neoplasm.