We used a cohort of ESCC tissues (SYSUCC, n = 108; clinicopathological information is provided in Supplementary Table 9) to analyze TMPO-AS1 expression using qPCR and to analyze TMPO, Ki67, CyclinD1, and MTA1 expression using IHC in order to collectively evaluate whether the TMPO-AS1/TMPO axis is clinically and pathologically relevant in ESCC. This evidence concerns the gene MKI67 and esophageal squamous cell carcinoma.