A qPCR array containing 12 genes associated with G1/S phase transition and 89 metastasis-related gene probes28 (Supplementary Table 8) was used to compare the mRNA expression profiles between TMPO-AS1-knockdown cells and control cells as well as between TMPO-knockdown cells and control cells as an approach to further identify downstream factors of TMPO-AS1 and TMPO involved in ESCC cell proliferation and metastasis. This evidence concerns the gene TMPO and esophageal squamous cell carcinoma.