VHL and von Hippel-Lindau disease: As expected, wild-type HA-VHL and Chuvash HA-VHLR200W mutant bound similarly to the dihydroxy-TFAM peptide (P-OH-53/66), but not to any VHL syndrome mutants (Fig. 4j and Extended Data Fig. 4i), confirming the IVT 35S-VHL capture assay (Fig. 4i).