RPS6KB1 and non-small cell lung carcinoma: As shown, in stable pCan-1 primary NSCLC cells with SLC5A3 shRNA (shSLC5A3-S1, shSLC5A3-S2) or the lenti-CRISPR/Cas9-SLC5A3-KO construct (koSLC5A3), levels of phosphorylated Akt and S6K1 were significantly decreased (Fig. 6A).