To support that SLC5A3-driven NSCLC cell progression was associated with Akt-mTOR activation, an adenoviral constitutively-active Akt1 (“caAkt1”, S473D) was transduced to koSLC5A3 pCan-1 cells, and stable cells were established after selection. The gene discussed is SLC5A3; the disease is non-small cell lung carcinoma.