In different patient-derived primary NSCLC cells and immortalized lines, SLC5A3 silencing (using targeted shRNAs) or KO (by the CRSIPR/Cas9 method) robustly impeded cell viability, proliferation and migration in vitro, and provoking G1-S arrest and apoptosis. The gene discussed is SLC5A3; the disease is non-small cell lung carcinoma.