Blotting for ICP27, TK, and VP16, representative of the viral gene classes IE, E, and L respectively, showed that the kinetics of expression of all three classes was similar between WT and HSV1 vhs-GFP infections, while vhs and GFP blots confirmed the expression of vhs-GFP with similar kinetics to vhs alone (Fig. 3E). The gene discussed is TKT; the disease is infection.