To confirm the targeting relationship of these two genes, the dual-luciferase reporter gene system assay was conducted, and we illustrated that miR-380-3p mimic significantly suppressed luciferase activities in the PC cells co-transfected with wild-type PTEN luciferase vectors, and the luciferase activities were enforced by the miR-380-3p inhibitor (Figure 3(b-c)), implying that miR-380-3p was capable of targeting the predicting sites. The gene discussed is PTEN; the disease is pachyonychia congenita.