For additional verification of the expression of AHSA1 in hepatocellular carcinoma, RT-PCR was performed to detect the mRNA expression levels of AHSA1 in normal hepatocyte cell line LO2 along with three hepatocellular carcinoma cell lines, HCCLM3, HepG2 and Huh7. Here, AHSA1 is linked to hepatocellular carcinoma.