Likewise, biochemical variables associated with ALT will be adjusted for in the Cox regression model, waist circumference, triglyceride, SBP, DBP, fasting glucose HbA1c; very low and negative correlation with age, HDL and eGFR, BMI, waist circumference, triglyceride, SBP, DBP, fasting glucose HbA1c, age, HDL, and eGFR will be controlled for in the Cox regression model when assessing the association between ALT and metabolic disease. The gene discussed is GPT; the disease is metabolic disease.