BCR and chronic myelogenous leukemia, BCR-ABL1 positive: Therefore, we sought to devise a non-denaturing cell lysis method capable of protecting the critical targets in CML primary leukocytes, predominantly in the protease-rich fraction represented by myeloid cells, from proteolysis (in this specific case including, but not restricted to, BCR::ABL1), followed by a specific kinase assay.