In this study, our results showed that gene fusion within chromosomes mainly occurred in bone tumors, and the gene fusion is inclined to occur on chromosome 17 and chromosome 12, including the fusion of TP53, ETV6, and KMT2D. Although Ewing’s sarcoma is not considered to be included in osteosarcoma (41), the identification of fusion in osteosarcoma is still useful for auxiliary diagnosis. Here, TP53 is linked to osteosarcoma.