Here, we found that overexpression of miR-4800-3p could greatly suppress E-Cad and ZO-1 protein levels but increased the progression of N-Cad in both in vitro and in vivo experiments, demonstrating that miR-4800-3p could weaken the ability of invasion and metastasis of HCC cells. The gene discussed is TJP1; the disease is hepatocellular carcinoma.