Among the other mutations found in the copy number analysis, a mutation located on chromosome (chr)3 in Ff-I14s04 ABII KO #2 might have been included in a possible CIITA gRNA off-target region, which was defined by an interval of 1 kbp around the sequences, allowing for 5 bp of mismatches and/or gaps and including the RPN1 gene, which was listed among known cancer-related genes (referred to in COSMIC census version 88, https://cancer.sanger.ac.uk/cosmic/census, Table S4, Data S1 and S2). The gene discussed is RPN1; the disease is cancer.