Among the identified small mutations, including SNVs and indels, which may affect amino acid sequences, the genome-edited iPSCs that we generated in this study did not show mutations either in cancer-related genes other than the target genes (including HLA-A, HLA-B, and CIITA) or in the neighboring predicted off-target sequences (Table S5; Data S1 and S2). Here, HLA-B is linked to cancer.