Based on the abovementioned findings, quantitative reverse-transcription polymerase chain reaction (qRT-PCR), western blot, Celigo high-content screening (HCS), exon sequencing, and functional experiments were used to screen and verify multiple functional downstream genes of THSD7A, including SCARA5. Through HCS experiments, we determined that SCARA5, as the downstream driving gene of THSD7A, has a critical role in promoting ESCC. Here, THSD7A is linked to esophageal squamous cell carcinoma.