BACE1 and hepatocellular carcinoma: We performed RT-PCR after the extraction of total RNA from tissues and found that three out of five high-risk (HR > 1, Figure 3B) NRLs (BACE1-AS, SNHG3, SNHG4) were more expressed in HCC tissues than in paracancerous tissues (all p < 0.01, Figures 13D–F).