By Reactome pathway analysis, expression of majority of genes of mitochondrial aminoacyl-tRNA-synthetases was downregulated in DCM hearts, including Aars2, Cars2, Farsb, Fars2, Hars2, Lars2, Mars2, Nars2, Ppa2, Rars2, Tars2, and Vars2 (Figure 3B). This evidence concerns the gene HARS2 and familial dilated cardiomyopathy.