To find out the role of hub genes in the overall survival of gastric cancer we performed a multivariate Cox regression analysis, among 21 hub genes, the eight genes (KCNJ3, CPVL, ONECUT2, SLC19A3, DDC, PRSS21, F12, and GRTP1) were designated as independent indicators of poor prognosis. This evidence concerns the gene PRSS21 and gastric cancer.