AKT1 and alkaline phosphatase measurement: Several previous studies implicated the PI3K/Akt pathway in trypsinogen activation, calcium overload, the nuclear translocation of NF-κB and cell apoptosis in the early course of AP, and strategies aim at PI3K inactivation to ameliorate the outcome of AP (Singh et al., 2001; Gukovsky et al., 2004; Abliz et al., 2015).