RALY has been considered to regulate colorectal cancer (CRC) cell proliferation and apoptosis as an RNA-binding protein (22) and play an important role in mRNA splicing and metabolism (9).To study the effect of RALY in PEDV infection, LLC-PK1 cells were transfected with FLAG-RALY plasmid and, after 24 h, infected with PEDV at an MOI of 1. This evidence concerns the gene RALY and colorectal cancer.