To corroborate the induction of apoptosis following single or combined treatments with AFA and GANT, the expression of Bax, Bcl-2, procaspase, and caspase 8/3, and cleavage of the poly (ADP-ribose) polymerase-1 (PARP-1) was analyzed by Western blotting in MM-treated cells (Fig. 5). This evidence concerns the gene CASP8 and Miyoshi myopathy.