SQSTM1 and Miyoshi myopathy: To demonstrate whether AFA 10 μM, GANT 20 μM and AFA 10 μM + GANT 20 μM treatments were able to induce autophagy in MM cell lines, the expression of proteins involved in the autophagic pathway, including Beclin-1, SQSTM-1/p62 and LC3-I/II was analyzed by Western blotting.