Recently, multi-omics for malignant ascites of GC revealed that disseminated GC was stratified into 2 groups, and 1 of them, with active EMT according to the expression profile, bearing transforming growth factor-β pathway activation through SMAD3 SE activation and high expression of the transcriptional enhancer factor TEF-1 [32]. This evidence concerns the gene SMAD3 and gastric cancer.