The data showed that PEG11as silencing inhibited IS/R-induced autophagy over-activation, along with reversing the increasing of MAP1LC3B/LC3B-II expressions, and the decreasing of SQSTM1/p62 expression induced by cerebral ischemia/R, hinting that knockdown of PEG11as expression inhibited the autophagic dysfunction. The gene discussed is SQSTM1; the disease is brain ischemia.