CD4 and neoplasm: From the tumor volume diagram of mice, we could determine that the anti-tumor efficacy of the combined vaccine was significantly better than that of the control group and triple adjuvant group, which indicated that the combination of the triple adjuvant and the whole-cell vaccine had a synergistic effect and could simultaneously expand CD4+ T cells and CD8+ T cells in the body, produce powerful anti-tumor efficacy and prolong the survival time of the mice (Fig. 3a–c).