We analyzed a MIBI triple-negative breast cancer (TNBC) dataset (Keren et al., 2018) studying the co-expression of two sets of functional markers, (a) HLA-DR, CD45RO, H3K27me3, H3K9ac and HLA-Class-1, and (b) PD1, PD-L1, Lag3 and IDO, which are also known as immuno-regulatory proteins (IRP’s). The gene discussed is CD274; the disease is triple-negative breast carcinoma.