In this study, we not only detected the effect of TBK1 on HCC cells in molecular experiments, cellular experiments, and animal experiments but we also detected the expression of TBK1 in clinical HCC tissue samples, confirming that TBK1 has clinical significance (Ou et al., 2011; Gao et al., 2021; Zhu et al., 2021). This evidence concerns the gene TBK1 and hepatocellular carcinoma.