In this study, we overexpressed and knocked down TBK1 in HCC cells, then detected the expression levels of various factors, including: 1) survivin and cIAP related to cell survival and apoptosis -1/2, or BCL-2; 2) epithelial-mesenchymal transition-related factors, such as vimentin and N-cadherin (markers of mesenchymal transition), and E-cadherin (marker of an epithelial phenotype); 3) P65 and P50 of NF-κB; and 4) NICD of Notch protein (Zhang Y et al., 2017). Here, VIM is linked to hepatocellular carcinoma.