AHSG and breast cancer: To further explore the effect of AHSG expression on the cell cycle of BC cells, flow cytometry was used for the detection of changes in the cell cycle of the T24 and 5637 cells after AHSG overexpression/knockdown, and we discovered that AHSG overexpression promoted the cell cycle from the G1 phase to the S phase in the T24 and 5637 cells (Figure 3(e-f)).