Furthermore, in multivariate analysis, a high density of PD-L1-expressing cells, a combination of high densities of PD-L1-expressing cells and Tregs, and a combination of the presence of deletion mutations spanning the pre-S2 gene segment and high densities of PD-L1-expressing cells and Tregs, were shown as independent biomarkers for predicting a higher risk of HCC recurrence (HR (95% CI), 2.663 (1.069–6.632), p value = 0.0354; 2.580 (1.022–6.516), p value = 0.0449; 3.163 (1.309–7.644), p value = 0.0105, respectively) (Table 3). This evidence concerns the gene CD274 and hepatocellular carcinoma.