CD274 and hepatocellular carcinoma: Moreover, a combination of the presence of deletion mutations spanning the pre-S2 gene segment and high densities of PD-L1-expressing cells and Tregs was significantly associated with a higher risk of HCC recurrence (HR (95% CI), 4.109 (1.763–9.572), p value = 0.0011) and poorer RFS (median (range), 8.2 (0.8–72.3) versus 18.0 (1.8–43.7) months, p value = 0.0004) than those of other combinations (Table 3 and Figure 1G).