The data showed that the rNS1 F9Y, N4T + L95H + E172A and D2I + F9Y significantly enhanced virus titers at the early stages of infection 12 (p < 0.001) and 24 hpi (p < 0.01) compared to wild type WSN in MDCK cells (Figure 2A). The gene discussed is RNASE1; the disease is infection.