CD80 and melanoma: More recently, Sainz et al. combined the NKT agonist α-GalCer, melanoma-associated peptide antigens as well as TLR ligands CpG and MPLA with nanoparticles, which promoted DC maturation, consequently leading to the up-regulation of co-stimulatory markers, such as CD80/CD86, and the production of IL-12/CCL17.