TotalSeq assay from mouse serum EVs revealed that TLM_CFM-F_OSM induced the downregulation of exosomal marker CD63, CD81, and oncogenic proteins (EGFR and PD-L1) in lung cancer, and we further validated these results by checking the protein expression through Western blotting in lung cancer tissues after their respective treatments. The gene discussed is EGFR; the disease is lung cancer.