TotalSeq assay from mouse serum EVs revealed that TLM_CFM-F_OSM induced the downregulation of exosomal marker CD63, CD81, and oncogenic proteins (EGFR and PD-L1) in lung cancer, and we further validated these results by checking the protein expression through Western blotting in lung cancer tissues after their respective treatments. This evidence concerns the gene CD81 and lung carcinoma.