The earliest reports of L-asparaginase discovered in bacteria showed that E. coli cells possess one or two asparaginase activities due to two distinct enzymes which differ in their affinities for L-asparagine, where the enzyme with the higher affinity (asparaginase II, located in the periplasmic region) markedly inhibited lymphomas in mice, while the asparaginase with the lower affinity (asparaginase I, found in the cytoplasm) was ineffective [9,10]. Here, ASPG is linked to lymphoma.