The research mechanism found that after grape derived EVs were ingested by intestinal stem cells, the number of BMI1 genes was increased; the gene expression of stem cell marker SOX2, Naong, OCT4, KLF4 was significantly up-regulated by encoding pluripotent stem cells; in addition, it triggered downstream normative Wnt signal activation, induced Lgr5+ stem cell proliferation, accelerated intestinal mucosal regeneration, promoted intestinal remodeling, protected mouse intestine and inhibited DSS-induced colitis. This evidence concerns the gene KLF4 and colitis.