The results show that Kaliziri extract can directly activate tyrosinase activity in B16 melanocytes and promote the melanin content and tyrosinase activity of B16 melanocytes; RT-PCR and western blot experiments further revealed that it can promote the upregulation of several genes and proteins closely associated with melanin synthesis in B16 melanoma cells, including MITF, TYR, TRP1, and TRP2. The gene discussed is TYR; the disease is melanoma.