We compared the individual cytokine expression levels at different time points and observed that compared to the wild type H37Rv, expression of IL-1β in Mtb∆2159 was increased by 1.5-folds on day 1, 3, and 5 post infection; IP-10 expression was increased by 1.5-folds on day 3; MIP-1 α expression increased by two-folds on day 1 and 3. This evidence concerns the gene IL1B and infection.