EEF1A1 and infection: The transfection system inserts DNA sequences into one of the two copies of Elongation Factor 1 alpha (EF1α) and has been used to express foreign proteins including green fluorescent protein [13], R. microplus BM86, or tick glutathione-S-transferase by transfected B. bovis during infection of the mammalian host [15], and to explore the mechanism of parasite erythrocyte invasion [16].