Our goal is to (1) determine the distribution of DoGo LNP-mediated systemically delivered siRNA in the ischemic brain, (2) establish the brain cell-type specificity of DoGo LNPs and (3) evaluate the effect of RNAi-mediated silencing of toll-like receptor 4 (TLR4) on the stroke-induced neuroinflammation. Here, TLR4 is linked to Stroke.