We discovered the novel mechanism that Alisol B upregulated the disrupted expression of RARα and then decreased CD36 expression via RARα-HNF4α-PPARγ transcriptional cascade, which further alleviated hepatic steatosis, oxidative stress, inflammation, and fibrosis in the liver of NASH mice. The gene discussed is HNF4A; the disease is metabolic dysfunction-associated steatohepatitis.