In contrast to CLDN-4, the CLDN-3 high-expression group was frequently located at the lower third compared to the CLDN-3 low-expression group (73.4% vs. 31.6%, p = 0.006); however, other variables such as gender, age, tumor size, and the presence of synchronous tumor were not significantly different between high and low expressions of CLDN-3. This evidence concerns the gene CLDN4 and neoplasm.