As a result of limited diagnostic potential using high-performance liquid chromatography (HPLC) [23], as it may not detect high oxygen affinity hemoglobinopathies, our NGS panel includes mutations in α- or β-globin genes (HBB, HBA1, HBA2) and the use of HPLC is not part of our algorithm. This evidence concerns the gene HBA1 and hemoglobinopathy.