TARDBP and amyotrophic lateral sclerosis: To this end, we have aimed to analyze how previously characterized optineurin truncation (Optn470T) [22], which mimics ALS mutations found in patients, or complete optineurin deficiency generated by the CRISPR/Cas-9 technology affects TDP-43 in primary microglia and related microglial cell line (BV2), respectively.