Then, as CBS overexpression has also been shown to stimulate cell proliferation in HCT116 colon cancer, and therapeutic manipulation of H2S has recently been proposed as an anticancer candidate [9,42,43], we assessed whether CQ, CHX and NHX may be able to decrease H2S production and consequently cell proliferation in HCT116 cells. The gene discussed is CBS; the disease is malignant colon neoplasm.