In parallel, we treated splenocytes from mice fed a normal diet using MBCD/Chol complexes ex vivo to ascertain that the reduction in P2X7 sensitivity of the CD4+ and CD8+ T cell subsets following the HFC diet was mainly dependent on plasma membrane cholesterol modulation and not the consequences of the modulation of various biological parameters linked to obesity. Here, CD8A is linked to obesity due to melanocortin 4 receptor deficiency.