The results of western blot analysis revealed that the levels of TP53, BAX and p21 were notably upregulated, whereas the BCL-2 level was significantly downregulated in EVA1A-overexpressing HCC cells, suggesting that EVA1A overexpression induced p53/BAX-mediated apoptosis, p53/p21-mediated cell cycle arrest, and TP53 was the downstream target of EVA1A inhibition of tumor. The gene discussed is EVA1A; the disease is hepatocellular carcinoma.