Flubendazole upregulated dynamin-related protein (DRP1) and p-DRP1Ser616 in MDA-MB-231 and MCF-7 cells, and the inhibition of DRP1 with shRNA or inhibitor mdivi-1 significantly reduced the expression of PINK1 and Parkin promoted by flubendazole, indicating that DRP1 mediates flubendazole-promoted mitophagy in breast cancer. The gene discussed is PRKN; the disease is breast carcinoma.