To explore the effect of TRX2 on colorectal cancer (CRC) cells, we overexpressed or knocked down TRX2 in HCT116 cells and evaluated the efficiency of overexpression plasmid FLAG-TRX2 and the siRNA targeting TRX2 by detecting the cell lysis (Figure 1A–C). This evidence concerns the gene TXN2 and colorectal carcinoma.